华美资讯

区分不同状态的胚胎干细胞新方法——介电电泳

发表时间:2013-01-09 09:14

当胚胎干细胞是在第一阶段,它有可能长成任何类型的细胞,科学家们称之为未分化的状态。现在一个来自苏格兰的科研小组研究展示了一种将晚期干细胞与未分化胚胎干细胞区分开来的新方法,研究结果发表在美国物理研究所(AIP)杂志Biomicrofluidics上。

研究人员利用介电泳(dielectrophoresis)方法分离干细胞。他们根据不同的频率所用的电压产生的电场研究细胞如何移动,由细胞的电性能的影响的响应。结果发现,已分化的干细胞外膜可以储存更多的电荷,明显区别于未分化的细胞。

研究人员表示,这或者是因为已分化的干细胞形态发生变化如起皱,折叠,细胞的膜变薄,因而可存储更多的电荷。本次研究有利于需要分离细胞的研究和疾病如帕金森氏的治疗。

原文摘要:

Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives

Assessment of the dielectrophoresis (DEP) cross-over frequency (fxo), cell diameter, and derivative membrane capacitance (Cm) values for a group of undifferentiated human embryonic stem cell (hESC) lines (H1, H9, RCM1, RH1), and for a transgenic subclone of H1 (T8) revealed that hESC lines could not be discriminated on their mean fxo and Cmvalues, the latter of which ranged from 14 to 20 mF/m2. Differentiation of H1 and H9 to a mesenchymal stem cell-like phenotype resulted in similar significant increases in meanCm values to 41–49 mF/m2 in both lines (p < 0.0001). BMP4-induced differentiation of RCM1 to a trophoblast cell-like phenotype also resulted in a distinct and significant increase in mean Cm value to 28 mF/m2 (p < 0.0001). The progressive transition to a higher membrane capacitance was also evident after each passage of cell culture as H9 cells transitioned to a mesenchymal stem cell-like state induced by growth on a substrate of hyaluronan. These findings confirm the existence of distinctive parameters between undifferentiated and differentiating cells on which future application of dielectrophoresis in the context of hESC manufacturing can be based.